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Rats were housed in a humidity- (50 5%) and temperature-controlled (23 2) room, under a 12/12 h light (200 lux)/dark cycle, with rodent standard chow and water available ad libitum

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the final sample size was determined based on feasibility

and v) drought stress with combined G5 and Si application (D+G5+Si) group, in which plants were watered to keep 35%45% of the saturation moisture content and both G5 (10 8 CFU/ml) and Si (2 mM) were applied

Travell and Simon emphasized in their book titled, Myofascial Pain and Dysfunction: Upper half of body that an inquiry into the nature of sleep is important in MPS patients.[56] They stated that anxious and depressed patients have a trouble falling asleep