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M.NowickyJ

doi: 10.1016/j.chembiol.2019.01.008 78

doi: 10.1016/j.jare.2018.05.012

Ice crystal formation disrupts the peptides structure, and repeated freeze-thaw cycles have been observed to reduce biological activity by 20-50% (2)

Swisher SG , Roth JA, Lawrence DD, Kemp BL, Carrasco CH, Fossella FV, Glisson BS, Hong WK, Khuri FR, Kurie JM, Nesbitt JC, Pisters KM, Putnam JB, Schrump DS, Shin DM, Walsh GL