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Afterward, the samples were lysed and centrifuged (12,000g, 4 C), after which the supernatants were collected

doi: 10.1016/j.toxlet.2015.06.001 21 WuXCiminieriCBosISTWoestMEDAmbrosiAWardenaarRet al

Moldoveanu B, Otmishi P, Jani P et al (2009) Inflammatory mechanisms in the lung

It was developed in the 1990s by Novo Nordisk and studied extensively in clinical trials for GH deficiency and wasting conditions, though it was ultimately not commercialized in favor of other compounds